The refectory of the convent of Ognissanti is famous for the large fresco painted in 1488 by Domenico Ghirlandaio. Besides the Last Supper fresco and its sinopia, the refectory also preserves a fine doorway and a lavabo , both dated 1488, and an Annunciation of the early 15th century.

The cloister leading to the refectory has frescoes of the Life of St Francis dating from the early 17th century by Florentine artists, including Jacopo Ligozzi and Giovanni da San Giovanni.